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How can I determine plant extract concentration?

I understand that you want to prepare a stock solution (s.s.) concentration from the plant extract to be examined by dilutions in a final concentration with keeping the percent of

How do you determine the concentration of plant extract?

For the determination of extract concentration you need to evaporate the solvent (by vacuum evaporator or lyophilisator) and than dissolve the crude extract in the known

Determination of molar concentration of plant extract

If you have a crude plant extract containing a mixture of substances, the concept of molar concentration is meaningless unless you are interested in one single compound in that

How can we determine the concentration of an extract

Here the word concentration is not suitable but it would be considered 100% plant extract or herbal extract. It still retains some of the solvent (3-1%) which is difficult to be removed.

2.0 MATERIALS AND METHODS 2.1 Plant material and

2013-2-27  A stock solution of plant aqueous extract was prepared by dissolving 100 mg of extract in 1 ml of sterile distilled water. Likewise, for the ethanol extract, 100 mg of extract was dissolved in 1 ml of 10 % Tween-20 rather than the original solvent (i.e. Dimethyl sulfoxide), and so the initial concentration of the plant extract (100 mg/ml)

Relative efficacy of three medicinal plant extracts in the

2002-7-1  Relative importance of Bacopa monnieri (200 mg/kg), Aegle marmelos (1.00 g/kg) and Aloe vera (125 mg/kg) leaf extracts in the regulation of thyroid hormone concentrations in male mice was investigated. While serum levels of both T 3 and T 4 were inhibited by A. vera, A. marmelos extract could decrease only T 3 concentration. On the other hand, T 4 concentration was increased by B. monnieri

Antimicrobial activity of some plant extracts against

2018-2-1  All plant extracts was potentially effective against S. typhi, E. coli, B. cereus and S. aureus implicated in food spoilage but the extract of Punica granatum was the most effective extract with concentration of 500 mg/ml. Ethanolic P. granatum peels extracts was found to be potentially effective against Micrococcus luteus, S. aureus, Bacillus

Protocol: Extract bioactive substances from plants a

2021-8-31  threat. A lot of plants contain toxic compounds. Even with low concentration of compounds occurring in the original plant material, be aware of ''sola dosis facit venenum'' the dose makes the poison. Sometimes an extract or even the working process can be extremly dangerous due to the high concentration of substance.

(PDF) Plant...Extraction ResearchGate

Grinding of plant materials. Making of fine powder with mechanical grinder. Small particles (10µm) increase the surface area. for extraction efficiency. For the extraction of dried powdered plant

Synthesis and characterization of ZnO nanoparticles using

2021-2-12  During synthesis, there are three factors/variables (Zinc acetate, plant extract concentration and pH), while the response is absorption maximum of ZnO NPs. First plant extract (1–5%) is immersed to zinc acetate solution (1–5 mM) in a conical flask and the pH of the mixed solution is adjusted to pH (3, 7, 11) using 0.1 M HCl/0.1 M NaOH

Effects of Varying Concentrations of the Crude Aqueous

2009-3-7  Plant material residues were gently dried in the oven at 40 °C to stable weights. The amount of plant material in extract was expressed as a percentage of the original dry weight of powdered plant material (% Yields: Hot water extracts, 13.34 ± 0.13 23.56 ±

Antibacterial Effects of Different Concentrations of

In addition, the antibacterial effect of the extract increased with an increase in its concentration. The extract exerted a greater antibacterial effect on S. mutans than on L. acidophilus. The plant polyphenols content is 3.7% which is equivalent to 29.93 g/ml.

Efficacy of different concentrations of garlic extract in

Background: Garlic is a plant with antimicrobial effects and different concentrations of garlic extract can decrease oral microorganisms. This study evaluates the effectiveness of different concentrations of garlic extract in an oral salivary microbial population.

2.0 MATERIALS AND METHODS 2.1 Plant material and

2013-2-27  A stock solution of plant aqueous extract was prepared by dissolving 100 mg of extract in 1 ml of sterile distilled water. Likewise, for the ethanol extract, 100 mg of extract was dissolved in 1 ml of 10 % Tween-20 rather than the original solvent (i.e. Dimethyl sulfoxide), and so the initial concentration of the plant extract (100 mg/ml)

Evaluation of diffusion and dilution methods to determine

The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) was determined for evaluating the potential plant extract. The antibacterial results showed methanol extracts (0.4 g/ml) of Ocimum gratissimum and Ocimum sanctum showed maximum zone of inhibition (30 mm and 25.5 mm, respectively) against Salmonella typhi .

Relative efficacy of three medicinal plant extracts in the

2002-7-1  Relative importance of Bacopa monnieri (200 mg/kg), Aegle marmelos (1.00 g/kg) and Aloe vera (125 mg/kg) leaf extracts in the regulation of thyroid hormone concentrations in male mice was investigated. While serum levels of both T 3 and T 4 were inhibited by A. vera, A. marmelos extract could decrease only T 3 concentration. On the other hand, T 4 concentration was increased by B. monnieri

Protocol: Extract bioactive substances from plants a

2021-8-31  threat. A lot of plants contain toxic compounds. Even with low concentration of compounds occurring in the original plant material, be aware of ''sola dosis facit venenum'' the dose makes the poison. Sometimes an extract or even the working process can be extremly dangerous due to the high concentration of substance.

Dose filtering a plant extract with 0.45 µm syringe filter

Dose filtering a plant extract with 0.45 µm syringe filter reduce the concentration of phytochemicals? Dose UV irradiation changes the chemical structure of phytochemicals in a plant compound?

Physiological and Biochemical Effects of an Aqueous

2021-9-14  The VI data from the present study further underlines the effectiveness of duckweed extract at a concentration of 0.50% as a plant biostimulant. The three highest dosages of the duckweed extract caused a general increase in leaf area (Fig. 3 a), in line with other studies that have found that biostimulants or vegetal plant extracts can

Techniques for extraction and isolation of natural

2018-4-17  Concentration of phenolic compounds in aqueous mate (Ilex paraguariensis A. St. Hil) extract through nanofiltration. LWT Food Sci Technol. 2011; 44 (10):2211–2216. [ Google Scholar ]

Evaluating Poplar Leaf Extract Concentration on Potential

2017-6-9  Evaluating Poplar Leaf Extract Concentration on Potential Plant Pathogens Jalaj Saxena1*, Vijay Vardhan Pandey1 and Reenu Sisodia2 1Forest Pathology Division, Forest Research Institute, Dehradun, 248006, Uttrakhand, India 2Department of Forestry, Dr. B.R. Ambedkar University, Agra, 282002, Uttar Pradesh, India

A sensitive and quick microplate method to determine the

A sensitive and quick microplate method to determine the minimal inhibitory concentration of plant extracts for bacteria Planta Med . 1998 Dec;64(8):711-3. doi: 10.1055/s-2006-957563.

Effects of Varying Concentrations of the Crude Aqueous

2009-3-7  Plant material residues were gently dried in the oven at 40 °C to stable weights. The amount of plant material in extract was expressed as a percentage of the original dry weight of powdered plant material (% Yields: Hot water extracts, 13.34 ± 0.13 23.56 ±

Antibacterial Effects of Different Concentrations of

In addition, the antibacterial effect of the extract increased with an increase in its concentration. The extract exerted a greater antibacterial effect on S. mutans than on L. acidophilus. The plant polyphenols content is 3.7% which is equivalent to 29.93 g/ml.

TOTAL PHENOLIC CONTENT, FLAVONOID

2011-6-22  The concentration of phenolics in plant extracts was determined using spectrophotometric method (SINGLETON et al., 1999). Methanolic solution of the extract in the concentration of 1 mg/ml was used in the analysis. The reaction mixture was prepared by mixing 0.5 ml of methanolic solution of extract, 2.5 ml of 10% Folin-Ciocalteu’s reagent

Efficacy of different concentrations of garlic extract in

Background: Garlic is a plant with antimicrobial effects and different concentrations of garlic extract can decrease oral microorganisms. This study evaluates the effectiveness of different concentrations of garlic extract in an oral salivary microbial population.

Protocol: Extract bioactive substances from plants a

2021-8-31  threat. A lot of plants contain toxic compounds. Even with low concentration of compounds occurring in the original plant material, be aware of ''sola dosis facit venenum'' the dose makes the poison. Sometimes an extract or even the working process can be extremly dangerous due to the high concentration of substance.

Medicinal Plant Leaf Extract and Pure Flavonoid Mediated

2017-11-20  On increasing the concentration of plant extract into AgNO 3 solution, a red shift in the absorption band to the longer wavelength (from 430 to 455 nm in case of Tulsi extract

Dose filtering a plant extract with 0.45 µm syringe filter

Dose filtering a plant extract with 0.45 µm syringe filter reduce the concentration of phytochemicals? Dose UV irradiation changes the chemical structure of phytochemicals in a plant compound?

Physiological and Biochemical Effects of an Aqueous

2021-9-14  The VI data from the present study further underlines the effectiveness of duckweed extract at a concentration of 0.50% as a plant biostimulant. The three highest dosages of the duckweed extract caused a general increase in leaf area (Fig. 3 a), in line with other studies that have found that biostimulants or vegetal plant extracts can